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pUAS-hopscotch
(Plasmid #37301)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37301 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUAST
  • Backbone size w/o insert (bp) 8904
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hopscotch
  • Alt name
    hop
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    5100
  • Entrez Gene
    hop (a.k.a. Dmel_CG1594, 4, CG1594, Dm JAK, DmHD-160, Dmel\CG1594, HD-160, HOP, Hop, Hop1, Hop2, Hpo, JAK, Jak, L4, Tum, Tum-1, d-jak, hop/JAK, jak, l(1)10Be, l(1)G18, l(1)L4, l(1)hop, msvl)
  • Promoter Hsp70

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer pUAST-5 (5'-CTGCAACTAAAATCTGCCAAGAAG-3')
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Perrimon Lab plasmid #100

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUAS-hopscotch was a gift from Norbert Perrimon (Addgene plasmid # 37301 ; http://n2t.net/addgene:37301 ; RRID:Addgene_37301)

  • For your References section:

    Activation of a Drosophila Janus kinase (JAK) causes hematopoietic neoplasia and developmental defects. Harrison DA, Binari R, Nahreini TS, Gilman M, Perrimon N. EMBO J. 1995 Jun 15;14(12):2857-65. PubMed 7796812
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