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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 37277 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepYUB1049
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Backbone manufacturerGhader Bashiri
- Backbone size (bp) 4921
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Vector typeBacterial Expression ; mycobacteria shuttle vector
- Promoter T7
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Tags
/ Fusion Proteins
- His-tag
- His-tag
Growth in Bacteria
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Bacterial Resistance(s)Hygromycin, 200 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid contains 2 His tags within the multiple cloning site. Please see plasmid map from depositing laboratory above for details.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYUB28b was a gift from Ted Baker (Addgene plasmid # 37277 ; http://n2t.net/addgene:37277 ; RRID:Addgene_37277) -
For your References section:
Metabolic engineering of cofactor F420 production in Mycobacterium smegmatis. Bashiri G, Rehan AM, Greenwood DR, Dickson JM, Baker EN. PLoS One. 2010 Dec 29;5(12):e15803. 10.1371/journal.pone.0015803 PubMed 21209917
Map uploaded by the depositor.
