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Addgene

pEF1a-Foxd3-ER
(Plasmid #37270)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37270 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF-IRES-hrGFP
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 8500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Foxd3
  • Alt name
    Hfh2
  • Alt name
    Genesis
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2500
  • GenBank ID
    1347473
  • Entrez Gene
    Foxd3 (a.k.a. CWH3, Gen, Genesis, Hfh2)
  • Promoter EF1
  • Tag / Fusion Protein
    • ER (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer none
  • 3′ sequencing primer none
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The ER tag was generated by PCR.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF1a-Foxd3-ER was a gift from Patricia Labosky (Addgene plasmid # 37270 ; http://n2t.net/addgene:37270 ; RRID:Addgene_37270)
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