pEF1a-Foxd3-ER
(Plasmid
#37270)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 37270 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEF-IRES-hrGFP
- Backbone size w/o insert (bp) 6000
- Total vector size (bp) 8500
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFoxd3
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Alt nameHfh2
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Alt nameGenesis
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SpeciesM. musculus (mouse)
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Insert Size (bp)2500
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GenBank ID1347473
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Entrez GeneFoxd3 (a.k.a. CWH3, Gen, Genesis, Hfh2)
- Promoter EF1
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Tag
/ Fusion Protein
- ER (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site Xho1 (not destroyed)
- 5′ sequencing primer none
- 3′ sequencing primer none (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The ER tag was generated by PCR.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEF1a-Foxd3-ER was a gift from Patricia Labosky (Addgene plasmid # 37270 ; http://n2t.net/addgene:37270 ; RRID:Addgene_37270)
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/78/94/aab309e8-9d43-11e1-ad04-003048dd6500.jpeg.940x940_q85_autocrop.png)