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Addgene

Foxd3-pIRES2 eGFP
(Plasmid #37269)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37269 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES2-EGFP
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5300
  • Total vector size (bp) 7800
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Foxd3
  • Alt name
    Hfh2
  • Alt name
    Genesis
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2000
  • GenBank ID
    1347473
  • Entrez Gene
    Foxd3 (a.k.a. CWH3, Gen, Genesis, Hfh2)
  • Promoter CMV
  • Tag / Fusion Protein
    • IRES-GFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer none
  • 3′ sequencing primer none
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Foxd3-pIRES2 eGFP was a gift from Patricia Labosky (Addgene plasmid # 37269 ; http://n2t.net/addgene:37269 ; RRID:Addgene_37269)
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