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Addgene

pCS2mFoxd3
(Plasmid #37267)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37267 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2
  • Backbone size w/o insert (bp) 4095
  • Total vector size (bp) 5500
  • Vector type
    Mammalian Expression ; Overexpression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Foxd3
  • Alt name
    Hfh2
  • Alt name
    Genesis
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2075
  • GenBank ID
    1347473
  • Entrez Gene
    Foxd3 (a.k.a. CWH3, Gen, Genesis, Hfh2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer none
  • 3′ sequencing primer none
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note the insert is comprised of the 1.4kb ORF surrounded by UTRs.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2mFoxd3 was a gift from Patricia Labosky (Addgene plasmid # 37267 ; http://n2t.net/addgene:37267 ; RRID:Addgene_37267)

  • For your References section:

    FoxD3 regulation of Nodal in the Spemann organizer is essential for Xenopus dorsal mesoderm development. Steiner AB, Engleka MJ, Lu Q, Piwarzyk EC, Yaklichkin S, Lefebvre JL, Walters JW, Pineda-Salgado L, Labosky PA, Kessler DS. Development. 2006 Dec;133(24):4827-38. Epub 2006 Nov 8. 10.1242/dev.02663 PubMed 17092955
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