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Addgene

Ch-gelsolN
(Plasmid #37262)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37262 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    EYFP-N1
  • Backbone manufacturer
    Clontech
  • Total vector size (bp) 6000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gelsolin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1144
  • Mutation
    deleted amino acids 380-731
  • Entrez Gene
    GSN (a.k.a. ADF, AGEL)
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ch-gelsolN was a gift from Ikuo Wada (Addgene plasmid # 37262 ; http://n2t.net/addgene:37262 ; RRID:Addgene_37262)

  • For your References section:

    Regulated motion of glycoproteins revealed by direct visualization of a single cargo in the endoplasmic reticulum. Nagaya H, Tamura T, Higa-Nishiyama A, Ohashi K, Takeuchi M, Hashimoto H, Hatsuzawa K, Kinjo M, Okada T, Wada I. J Cell Biol. 2008 Jan 14;180(1):129-43. 10.1083/jcb.200704078 PubMed 18195104
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