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Addgene

PBMN YFP-ER50
(Plasmid #37261)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37261 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBMN iHcRed
  • Backbone manufacturer
    Gary Nolan
  • Backbone size w/o insert (bp) 6940
  • Total vector size (bp) 8453
  • Vector type
    Retroviral
  • Selectable markers
    HcRed Fluorescence

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ER50
  • Alt name
    ERLBD
  • Alt name
    ERS1 ligand binding domain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    750
  • Mutation
    6 mutations, T371A, L384M, M421G, G521R, Y537S, N519S
  • GenBank ID
    BC128573.1
  • Entrez Gene
    ESR1 (a.k.a. ER, ESR, ESRA, ESTRR, Era, NR3A1)
  • Promoter LTR
  • Tag / Fusion Protein
    • HA-YFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NsiI (not destroyed)
  • 5′ sequencing primer ATCGCAGCTTGGATACACGCC
  • 3′ sequencing primer GAGAGGGGCGGAATTTACGTAGC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There is a G insertion at 1651 compared to the depositor's sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PBMN YFP-ER50 was a gift from Thomas Wandless (Addgene plasmid # 37261 ; http://n2t.net/addgene:37261 ; RRID:Addgene_37261)

  • For your References section:

    Destabilizing domains derived from the human estrogen receptor. Miyazaki Y, Imoto H, Chen LC, Wandless TJ. J Am Chem Soc. 2012 Mar 7;134(9):3942-5. Epub 2012 Feb 22. 10.1021/ja209933r PubMed 22332638
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