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pLK69
(Plasmid #37258)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37258 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAdLox
  • Backbone size w/o insert (bp) 4224
  • Vector type
    Mammalian Expression, Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    vang-like 2
  • Alt name
    vangl2; LPP1; LTAP; STB1; STBM; STBM1; KIAA1215; MGC119403; MGC119404
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1553
  • Mutation
    lacks the 4 C-term aa
  • GenBank ID
    NM_020335.2
  • Entrez Gene
    VANGL2 (a.k.a. LPP1, LTAP, STB1, STBM, STBM1)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site MfeI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer SV40pA-Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

contains EGFP hVangl2 h delta4

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLK69 was a gift from Ann Hubbard (Addgene plasmid # 37258 ; http://n2t.net/addgene:37258 ; RRID:Addgene_37258)

  • For your References section:

    Scribble associates with two polarity proteins, Lgl2 and Vangl2, via distinct molecular domains. Kallay LM, McNickle A, Brennwald PJ, Hubbard AL, Braiterman LT. J Cell Biochem. 2006 Oct 1;99(2):647-64. 10.1002/jcb.20992 PubMed 16791850
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