pLK25
(Plasmid
#37255)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 37255 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAdLox GI 1488684
- Backbone size w/o insert (bp) 4224
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameScribble
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Alt nameCRIB1; SCRB1; SCRIB1; Vartul
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SpeciesH. sapiens (human)
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Insert Size (bp)1425
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Mutationaa 727-1630
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GenBank ID23513
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Entrez GeneSCRIB (a.k.a. CRIB1, SCRB1, SCRIB1, Vartul, oSCRIB)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site MfeI (not destroyed)
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer SV40pA-Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
contains EGFP PDZ
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLK25 was a gift from Ann Hubbard (Addgene plasmid # 37255 ; http://n2t.net/addgene:37255 ; RRID:Addgene_37255) -
For your References section:
Scribble associates with two polarity proteins, Lgl2 and Vangl2, via distinct molecular domains. Kallay LM, McNickle A, Brennwald PJ, Hubbard AL, Braiterman LT. J Cell Biochem. 2006 Oct 1;99(2):647-64. 10.1002/jcb.20992 PubMed 16791850