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Addgene

pLK45
(Plasmid #37250)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37250 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pAdLox GI 1488684
  • Backbone size w/o insert (bp) 4224
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Scribble
  • Alt name
    CRIB1; SCRB1; SCRIB1; Vartul
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4890
  • GenBank ID
    23513
  • Entrez Gene
    SCRIB (a.k.a. CRIB1, SCRB1, SCRIB1, Vartul, oSCRIB)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XmaI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer SV40pA-Rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

contains EGFP-hScrib WT

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLK45 was a gift from Ann Hubbard (Addgene plasmid # 37250)

  • For your References section:

    Scribble associates with two polarity proteins, Lgl2 and Vangl2, via distinct molecular domains. Kallay LM, McNickle A, Brennwald PJ, Hubbard AL, Braiterman LT. J Cell Biochem. 2006 Oct 1;99(2):647-64. 10.1002/jcb.20992 PubMed 16791850
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