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Addgene

pGEX-5X2-RalBP1
(Plasmid #37245)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37245 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-5X2
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 4973
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RalBP1
  • Species
    H. sapiens (human)
  • Entrez Gene
    RALBP1 (a.k.a. RIP1, RLIP1, RLIP76)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer GST-F
  • 3′ sequencing primer pGEX_3_primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-5X2-RalBP1 was a gift from Christopher Counter & David Kashatus (Addgene plasmid # 37245 ; http://n2t.net/addgene:37245 ; RRID:Addgene_37245)

  • For your References section:

    RALA and RALBP1 regulate mitochondrial fission at mitosis. Kashatus DF, Lim KH, Brady DC, Pershing NL, Cox AD, Counter CM. Nat Cell Biol. 2011 Aug 7;13(9):1108-15. doi: 10.1038/ncb2310. 10.1038/ncb2310 PubMed 21822277
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