pBABE-puro-hDrp1(K38A)
(Plasmid #37243)

• Depositing Labs: Christopher Counter, David Kashatus
• Publication: Kashatus et al Nat Cell Biol. 2011 Aug 7;13(9):1108-15. doi: 10.1038/ncb2310.

Backbone

• Vector backbone: pBABE Puro
• Backbone manufacturer: Addgene Plasmid #1764
• Backbone size w/o insert (bp): 5169
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Drp1
• Alt name: DNM1L
• Species: H. sapiens (human)
• Mutation: brain-specific variant 3 with K38A mutation
• Entrez Gene: DNM1L (a.k.a. DLP1, DRP1, DVLP, DYMPLE, EMPF, EMPF1, HDYNIV, OPA5)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE 3'

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry