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Addgene

pTYB2-PAB1
(Plasmid #37234)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37234 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTYB2
  • Backbone manufacturer
    NEB
  • Backbone size w/o insert (bp) 7474
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PAB1
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    PAB1 (a.k.a. YER165W)
  • Promoter T7
  • Tag / Fusion Protein
    • intein (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site SmaI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTYB2-PAB1 was a gift from Jon Lorsch (Addgene plasmid # 37234 ; http://n2t.net/addgene:37234 ; RRID:Addgene_37234)

  • For your References section:

    Multiple elements in the eIF4G1 N-terminus promote assembly of eIF4G1*PABP mRNPs in vivo. Park EH, Walker SE, Lee JM, Rothenburg S, Lorsch JR, Hinnebusch AG. EMBO J. 2011 Jan 19;30(2):302-16. Epub 2010 Dec 7. 10.1038/emboj.2010.312 PubMed 21139564
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