pET-DUET-GST-eIF4G1-His6, eIF4E Sequences (4)

Addgene Sequences:

Partial (4)

Partial Sequences from Addgene (4)

Addgene has sequenced portions of this plasmid for verification. The results are shown below. Leading primers are indicated on the first line of each sequence.

Analyze Sequence

GenBank

SnapGene

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> T7-term TAGTAGACGAGTCCATGTGCTGGCGTTCAAATTTCGCAGCAGCGGTTTCTTTACCAGACTCGAGTTACAA GGTGATTGATGGTTGAGGGTGTCTACCATTGGCACTGGAATGTGGAAAGAATTCCAAATGCCCGTCATCG GTTAATTTTAAAACTTGCTTGAATTTACCACCAATTCTCAATAGTGGTTCTTTGTCTTCAGATTTAGTCC ATAAGGCAAACTTGTTACCACCTTTTCTAATGCTTAAAACGACACCGTTAATTTGGGAGTCGTCTTCATC AATTGTTTCACCAATAACTGCTAGTAAAGTTCTTAGCCATAATTCATCAATATCAGCACCTTTTCCTCTA AGTTGGAAAGACCATTTACCACCTTTAGCATTGGCTTCATCTTCCCATTCAGGTCTAACGTCATTACGGA AGACGTGGTAATCTGATTTCAATGGTAGTTCGTGTGGCTCAGGAATATTTTGAATGATAGCCCAAAATTC TTCAACAGTTTGGAATGAAGTGACGGGACGTAATAGATCAGACCACGACTCAGATTTATCGACGGCTGGC TTTGTGTACCATAAAGTCCATTTGGTGTTCAATGGGTGCTTGACATCGAAGTGAGCACTGTCACTTAAAA CAGTCTTTGGAGTAGCTGTGGTATCATCGACTGAAACGTTTTCTTCAAACTTCTTGCTAACTTCTTCAAC GGACATATGTATATCTCCTTCTTATACTTAACTAATATACTAAGATGGGGAATTGTTATCCGCTCACAAT TCCCCTATAGTGAGTCGTATTAATTTCGATTATGCGGCCGTGTACAATACGATTACTTTC
Analyze Sequence

GenBank

SnapGene

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> pBRrevBam 5'-GGTGATGTCGGCGATATAGG-3' CACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAG ATATACCATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTG GAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAA AGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTC TATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAG ATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACT TTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATG TCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTT GTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAG CTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGGCCTTTGCAGGGCTGGCAAGC CACGTTTGGTGGTGGCGACCATCCTCCAAAATCGGATCTGGTTCCCGCG
Analyze Sequence

GenBank

SnapGene

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> eIF4G1-3 5'-TGAGGAAGAGGAGAGACAACG-3' CGTACGAACAACTCCAGTAATAGACACTCTTTCAGAAGGGACGCTCCTCCTGCATCAAAAGATAGCTTTA TCACAACCAGAACATATTCTCAAAGAAATAGCCAAAGGGCTCCTCCTCCAAAGGAAGAACCAGCTGCACC AACTTCTACCGCAACAAATATGTTCAGTGCATTAATGGGAGAAAGTGATGACGAAGAGCATCATCATCAT CATCATTGAAAGCTTGCGGCCGCATAATGCTTAAGTCGAACAGAAAGTAATCGTATTGTACACGGCCGCA TAATCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCATCTTAGTATATTA GTTAAGTATAAGAAGGAGATATACATATGTCCGTTGAAGAAGTTAGCAAGAAGTTTGAAGAAAACGTTTC AGTCGATGATACCACAGCTACTCCAAAGACTGTTTTAAGTGACAGTGCTCACTTCGATGTCAAGCACCCA TTGAACACCAAATGGACTTTATGGTACACAAAGCCAGCCGTCGATAAATCTGAGTCGTGGTCTGATCTAT TACGTCCCGTCACTTCATTCCAAACTGTTGAAGAATTTTGGGCTATCATTCAAAATATTCCTGAGCCACA CGAACTACCATTGAAATCAGATTACCACGTCTTCCGTAATGACGTTAGANCTGAATGGGAAGATGAAGCC AATGCTAAAGGTGGTAAATGGTCTTTCCAACTTAGAGGAAAAGGTGCTGATATTGATGAATTATGGCTAA
Analyze Sequence

GenBank

SnapGene

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> pGEX5 GCGTGGATCCATGACAGACGAAACTGCTCAGCCGACACAATCTGCTTCAAAGCAGGAATCTGCTGCTCTG AAACAGACAGGCGATGACCAGCAGGAATCTCAGCAACAACGTGGCTACACCAACTACAACAATGGTAGCA ACTATACCCAAAAGAAGCCTTACAACAGCAACAGACCTCATCAGCAAAGAGGTGGTAAATTTGGACCAAA CAGATATAACAACCGTGGCAACTATAATGGTGGCGGTAGTTTCAGAGGTGGACACATGGGAGCCAACAGC TCAAACGTGCCATGGACTGGTTACTATAATAACTACCCCGTTTACTACCAGCCCCAGCAAATGGCGGCCG CTGGTAGTGCCCCTGCTAATCCAATTCCTGTCGAAGAGAAGTCACCTGTTCCAACTAAGATAGAAATCAC TACCAAGTCTGGTGAACACTTAGATTTGAAAGAACAGCATAAAGCCAAGCTACAGTCTCAGGAAAGATCT ACTGTGTCTCCGCAACCAGAGTCAAAGTTAAAAGAAACTTCTGATTCTACTTCTACTTCTACTCCAACTC CTACCCCTTCCACTAATGACTCTAAGGCCAGTTCTGAAGAAAATATATCTGAAGCTGAAAAGACAAGAAG AAATTTCATCGAGCAAGTTAAACTTCGTAAAGCTGCTTTAGAAAAGAAGAGAAAGGAGCAACTTGAAGGT TCTAGTGGCAACAATAATATTCCAATGAAGACTACCCCAGAAAATGTGGAAGAGAAGGGATCGGACAAAC CTGAGGTAACCGAAAAAACCAAGCCTGCTGA

pET-DUET-GST-eIF4G1-His6, eIF4E Sequences (4)

Partial Sequences from Addgene (4)

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