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Addgene

pUC19-DAD4
(Plasmid #37224)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37224 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2686
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DAD4
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    DAD4 (a.k.a. YDR320C-A, HSK2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer M13pUC-F
  • 3′ sequencing primer M13-pUC-Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The sequence for DAD4 mRNA was taken from yeast.utgenome.org. Two G bases were added at the 5´-ends of the mRNA in order to promote efficient transcription and the sequence GGATC was added at the 3´-end as part of a BamHI site during cloning.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC19-DAD4 was a gift from Jon Lorsch (Addgene plasmid # 37224 ; http://n2t.net/addgene:37224 ; RRID:Addgene_37224)

  • For your References section:

    The 5'-7-methylguanosine cap on eukaryotic mRNAs serves both to stimulate canonical translation initiation and to block an alternative pathway. Mitchell SF, Walker SE, Algire MA, Park EH, Hinnebusch AG, Lorsch JR. Mol Cell. 2010 Sep 24;39(6):950-62. 10.1016/j.molcel.2010.08.021 PubMed 20864040
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