pMGAHisTev5B
(Plasmid #37221)

• Depositing Lab: Jon Lorsch
• Publication: Acker et al Methods Enzymol. 2007;430:111-45.

Backbone

• Vector backbone: pSKB3
• Modifications to backbone: Vector is a derivative of pET28a(+) that has the thrombin site mutated to a Tev protease site

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: eIF5B
• Alt name: FUN12
• Species: S. cerevisiae (budding yeast)
• Mutation: N-term truncation (contains aa 396-1002)
• Tag / Fusion Protein:
  • His (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry