pTYB2-eIF1A
(Plasmid
#37218)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 37218 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTYB2
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Backbone manufacturerNEB
- Backbone size w/o insert (bp) 7474
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameeIF1A
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Alt nameTIF11
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SpeciesS. cerevisiae (budding yeast)
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (unknown if destroyed)
- 3′ cloning site SmaI (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTYB2-eIF1A was a gift from Jon Lorsch (Addgene plasmid # 37218 ; http://n2t.net/addgene:37218 ; RRID:Addgene_37218) -
For your References section:
Reconstitution of yeast translation initiation. Acker MG, Kolitz SE, Mitchell SF, Nanda JS, Lorsch JR. Methods Enzymol. 2007;430:111-45. 10.1016/S0076-6879(07)30006-2 PubMed 17913637