ZO-1 shRNA 2072
(Plasmid
#37207)
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Depositing Labs
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 37207 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSUPER
- Backbone size w/o insert (bp) 3176
- Total vector size (bp) 3200
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Vector typeMammalian Expression, RNAi
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namedog ZO-1 shRNA
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Alt nameZO-1
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gRNA/shRNA sequenceGGACAAACATGCTTTATTA
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Speciescanine
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Entrez GeneTJP1 (a.k.a. ZO-1, ZO1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ZO-1 shRNA 2072 was a gift from James Anderson & Alan Fanning (Addgene plasmid # 37207 ; http://n2t.net/addgene:37207 ; RRID:Addgene_37207) -
For your References section:
ZO-1 stabilizes the tight junction solute barrier through coupling to the perijunctional cytoskeleton. Van Itallie CM, Fanning AS, Bridges A, Anderson JM. Mol Biol Cell. 2009 Sep;20(17):3930-40. Epub 2009 Jul 15. 10.1091/mbc.e09-04-0320 PubMed 19605556