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Addgene

ceOtub1
(Plasmid #37196)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37196 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pProEX HTa
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4751
  • Total vector size (bp) 5600
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Otub1 ce
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    855
  • Entrez Gene
    otub-1 (a.k.a. C25D7.8)
  • Promoter trc_promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer M13_pUC_rev_primer
  • 3′ sequencing primer pTrcHis_rev_primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ceOtub1 was a gift from Cynthia Wolberger (Addgene plasmid # 37196 ; http://n2t.net/addgene:37196 ; RRID:Addgene_37196)

  • For your References section:

    The mechanism of OTUB1-mediated inhibition of ubiquitination. Wiener R, Zhang X, Wang T, Wolberger C. Nature. 2012 Feb 22;483(7391):618-22. doi: 10.1038/nature10911. 10.1038/nature10911 PubMed 22367539
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