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Addgene

UbG75C
(Plasmid #37194)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37194 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PET3a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 4640
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Start culture has to be freshly grow 4 to 6 hrs until O.D. around 0.6. Store it in 4oC overnight. Then it will be used to grow culture for expression.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Ubiquitin G75C mutant
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    228
  • Mutation
    Glycine 75 changed to Cystine
  • Entrez Gene
    RBX1 (a.k.a. BA554C12.1, RNF75, ROC1)
  • Promoter T7
  • Tag / Fusion Protein
    • No tag

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UbG75C was a gift from Cynthia Wolberger (Addgene plasmid # 37194 ; http://n2t.net/addgene:37194 ; RRID:Addgene_37194)

  • For your References section:

    The mechanism of OTUB1-mediated inhibition of ubiquitination. Wiener R, Zhang X, Wang T, Wolberger C. Nature. 2012 Feb 22;483(7391):618-22. doi: 10.1038/nature10911. 10.1038/nature10911 PubMed 22367539
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