pSG5 EDD C2768A
(Plasmid #37192)

• Depositing Labs: Darren Saunders, Charles Watts
• Publication: Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-78. Epub 2002 May 13.

Backbone

• Vector backbone: pSG5
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 4225
• Total vector size (bp): 12652
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EDD C2768A
• Alt name: UBR5 C2768A
• Species: H. sapiens (human)
• Insert Size (bp): 8397
• Mutation: Changed Cysteine 2768 to Alanine; K503R (please see depositor comments below)
• Entrez Gene: UBR5 (a.k.a. DD5, EDD, EDD1, HYD)
• Promoter: SV40
• Tags / Fusion Proteins:
  • FLAG (N terminal on insert)
  • 6x His Tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: RsrII (unknown if destroyed)
• 3′ cloning site: SfuI (unknown if destroyed)
• 5′ sequencing primer: T7 Forward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Amanda Russell, Cancer Research Program, Garvan Institute of Medical Research, NSW, Australia

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The depositing lab has noted that mutation K503R shouldn’t be a problem as it is outside the main functional domains. There does appear to be a relatively common SNP at K503 in Latino populations so this might be a genuine SNP-

http://exac.broadinstitute.org/variant/8-103338864-C-T

https://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=rs571861064