pEGFP-C1 EDD
(Plasmid #37190)

• Depositing Labs: Darren Saunders, Charles Watts
• Publication: Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-78. Epub 2002 May 13.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4708
• Total vector size (bp): 13103
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EDD
• Alt name: UBR5
• Species: H. sapiens (human)
• Insert Size (bp): 8395
• Entrez Gene: UBR5 (a.k.a. DD5, EDD, EDD1, HYD)
• Promoter: CMV
• Tags / Fusion Proteins:
  • EGFP (C terminal on insert)
  • 6x His Tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ApaI (unknown if destroyed)
• 3′ cloning site: SacII (unknown if destroyed)
• 5′ sequencing primer: EGFP-C

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Amanda Russell, Cancer Research Program, Garvan Institute of Medical Research, NSW, Australia
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry