pCMV-Tag2B EDD
(Plasmid #37188)

• Depositing Labs: Darren Saunders, Charles Watts
• Publication: Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-78. Epub 2002 May 13.

Backbone

• Vector backbone: pCMV-Tag2B
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 4373
• Total vector size (bp): 12770
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EDD
• Alt name: UBR5
• Species: H. sapiens (human)
• Insert Size (bp): 8397
• Mutation: Please see Depositor Comments
• Entrez Gene: UBR5 (a.k.a. DD5, EDD, EDD1, HYD)
• Promoter: CMV
• Tags / Fusion Proteins:
  • FLAG (N terminal on insert)
  • 6x His Tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ApaI (unknown if destroyed)
• 3′ cloning site: SacII (unknown if destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: T7 Forward

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: Amanda Russell, Cancer Research Program, Garvan Institute of Medical Research, NSW, Australia
• Articles Citing this Plasmid:
  • 12 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene NGS results identified a K503R mutation within the UBR5 translation. There is no information on the functional consequence of this mutation