UQ6148
(Bacterial strain #37178)

• Depositing Lab: Gary Roberts
• Publication: Gary Roberts unpublished plasmids (unpublished)

Backbone

• Vector backbone: pUX3254 AND pUX2410
• Modifications to backbone: pUX3254 = pUX3010 reporter system with a deletion between the “c” and “d” binding motifs such that the individual binding motifs “a,” “b,” “c,” “d,” “e,” and “f” are spaced at 21-bp intervals. The “c – d” sequence is 5′-TTCGGGAAACCG*GGTTCGGGTTTCATG-3′ where the asterisk represents the junction.

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): E. Coli UQ5853
• Growth instructions: Copy number: pUX2410 High / pUX3254 Low
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: coxM1′::lacZ reporter system with RcoM binding sites "a-f" spaced at 21bp AND RcoM1 with C-term 6xHIS tag

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pEXT20-F1
• 3′ sequencing primer: pRcoM1bs-R

Resource Information

• Supplemental Documents:
  • Strains for production of RcoMBx-1 proteins and assay of in vivo activity

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX3254 (coxM1′::lacZ reporter system with binding sites “a-f” with a deletion between the “c” and “d” binding motifs such that the individual binding motifs “a,” “b,” “c,” “d,” “e,” and “f” are spaced at 21-bp intervals) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag).

Strain is CmR, pUX3254 is KanR and SpecR, pUX2410 is AmpR. Can be grown in Amp + Kan.

For strain usage see attached pdf.