UQ6139
(Bacterial strain #37177)

• Depositing Lab: Gary Roberts
• Publication: Gary Roberts unpublished plasmids (unpublished)

Backbone

• Vector backbone: pUX3242 and pUX2410
• Modifications to backbone: pUX3242 = pUX2996 reporter system with higher-affinity RcoM1 binding site at the “e” position (“e↑” binding site = 5′-TCCTACAGTTCACGCACGT-3′)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): E. Coli UQ5853
• Growth instructions: Copy number: pUX2410 High / pUX3242 Low
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: coxM1′::lacZ reporter system with higher-affinity RcoM1 binding site at the "e" position AND RcoM1 with C-term 6xHIS
• Species: Burkholderia xenovorans

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pEXT20-F1
• 3′ sequencing primer: pRcoM1bs-R

Resource Information

• Supplemental Documents:
  • Strains for production of RcoMBx-1 proteins and assay of in vivo activity

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX3242 (pUX2996 with a coxM1′::lacZ reporter system such that ...vector DNA – BamHI (unique site) – higher-affinity binding site at the “e” position (“e↑” binding site = 5′-TCCTACAGTTCACGCACGT-3′) – coxM1′(with unique SalI site)::lacZ-> tfd <-KanR (with 5′-end unique XhoI site) – vector DNA <-SpR...) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag).

The strain is CmR, pUX3242 is KanR and SpecR and pUX2410 is AmpR. Can be grown in Amp + Kan.

For strain usage see attached pdf.