UQ5874
(Bacterial strain
#37169)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
No maps are available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Bacterial Strain | 37169 | Bacteria in agar stab | 1 | $85 | |
Backbone
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Vector backbonepUX3008 AND pUX2410
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Modifications to backbonepUX3008 is pUX2996 with binding site "f"
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)E. Coli UQ5853
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Growth instructionsCopy number: pUX2410 High / pUX3008 Low
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Copy numberUnknown
Gene/Insert
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Gene/Insert namecoxM1′::lacZ reporter system with RcoM binding site "f" AND RcoM1 with C-term 6xHIS tag
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pEXT20-F1
- 3′ sequencing primer pRcoM1bs-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX3008 (coxM1′::lacZ reporter system with binding site “f”) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag).
Strain is CmR, pUX3008 is KanR and SpecR, pUX2410 is AmpR. Can be grown in Amp + Kan.
For strain usage see attached pdf.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
UQ5874 was a gift from Gary Roberts (Addgene plasmid # 37169)