UQ4861
(Bacterial strain #37163)

• Depositing Lab: Gary Roberts
• Publication: Kerby et al J Bacteriol. 2008 May;190(9):3336-43. Epub 2008 Mar 7.

Backbone

• Vector backbone: pUX2424

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ~1200-bp fragment from Burkholderia xenovorans LB400 cloned into pEXT20
• Species: Burkholderia xenovorans

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: NA
• 3′ sequencing primer: pRcoM1bs-R

Resource Information

• Supplemental Documents:
  • Strains for production of RcoMBx-1 proteins and assay of in vivo activity

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This strain contains Roberts lab plasmid pUX2424 which contains ~1200bp fragment from Burkholderia xenovorans LB400 with introduced terminal HindIII and SalI sites [such that HindIII – (<-rcoM1
– intergenic region – coxM1′->) – SalI] cloned into pEXT20. Suitable
template for amplification and mutagenesis.

For strain usage see attached pdf.