UQ4803
(Bacterial strain #37162)

• Depositing Lab: Gary Roberts
• Publication: Kerby et al J Bacteriol. 2008 May;190(9):3336-43. Epub 2008 Mar 7.

Backbone

• Vector backbone: pUX2410

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): E. Coli VJS6737
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: rcoM1 in pEXT20
• Species: Burkholderia xenovorans
• Tag / Fusion Protein:
  • 6xHIS (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pEXT20-F1

Resource Information

• Supplemental Documents:
  • Strains for production of RcoMBx-1 proteins and assay of in vivo activity

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This strain contains Roberts lab plasmid pUX2410 which is an pUX2396 derivative suitable for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag. Host strain is E. coli VJS6737.

For strain usage see attached pdf.