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Addgene

UQ4802
(Bacterial strain #37160)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Bacterial Strain 37160 Bacteria in agar stab 1 $85
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Backbone

  • Vector backbone
    pEXT20

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    E. Coli VJS6737
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rcoM1 in pEXT20
  • Species
    Burkholderia xenovorans

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pEXT20-F2
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This strain contains Roberts lab plasmid pUX2396 which contains an 831-bp EcoRI-HindIII fragment bearing Burkholderia xenovorans LB400 rcoM1 cloned into pEXT20. Suitable for expression of RcoM1
protein (untagged). Host strain is E. coli VJS6737.

For strain usage see attached pdf.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UQ4802 was a gift from Gary Roberts (Addgene plasmid # 37160)

  • For your References section:

    RcoM: a new single-component transcriptional regulator of CO metabolism in bacteria. Kerby RL, Youn H, Roberts GP. J Bacteriol. 2008 May;190(9):3336-43. Epub 2008 Mar 7. 10.1128/JB.00033-08 PubMed 18326575
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