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Addgene

pDR125
(Plasmid #37150)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37150 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFASTBAC1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4776
  • Total vector size (bp) 8757
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    lig4
  • Alt name
    ligase IV
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3981
  • Entrez Gene
    LIG4 (a.k.a. LIG4S)
  • Tag / Fusion Protein
    • H6 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer Polyhedrin-fwd
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Thomas Lindahl
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDR125 was a gift from Dale Ramsden (Addgene plasmid # 37150 ; http://n2t.net/addgene:37150 ; RRID:Addgene_37150)
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