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pENTR-E2F1-PIP3A
(Plasmid #37142)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37142 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR/D-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2580
  • Total vector size (bp) 4998
  • Vector type
    Gateway

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    E2F1-P!P3A
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    2418
  • Mutation
    I153, Y156, Y157 to Alanine
  • GenBank ID
    AE014297.2
  • Entrez Gene
    E2f1 (a.k.a. Dmel_CG6376, CG6376, DRTF1/E2F, DmE2F-1, Dmel\CG6376, Dp, E(Sev-CycE)3A, E(var)3-93E, E(var)3-95E, E(var)93E, E2F, E2F-1, E2F1, E2f, Evar(3)164, dE2F, dE2F1, dE2f, dE2f1, de2f1, def21, drosE2F1, e2f, e2f1, l(3)07172, l(3)j3B1, l(3)j3C2, l(3)rM729)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR-E2F1-PIP3A was a gift from Robert Duronio (Addgene plasmid # 37142 ; http://n2t.net/addgene:37142 ; RRID:Addgene_37142)

  • For your References section:

    Intrinsic negative cell cycle regulation provided by PIP box- and Cul4Cdt2-mediated destruction of E2f1 during S phase. Shibutani ST, de la Cruz AF, Tran V, Turbyfill WJ 3rd, Reis T, Edgar BA, Duronio RJ. Dev Cell. 2008 Dec;15(6):890-900. 10.1016/j.devcel.2008.10.003 PubMed 19081076
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