pENTR-E2F1
(Plasmid
#37141)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 37141 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepENTR/D-TOPO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2580
- Total vector size (bp) 4998
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Vector typeGateway
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameE2F1
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Alt namedE2F
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Alt namedE2F1
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Alt nameE2F-1
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SpeciesD. melanogaster (fly)
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Insert Size (bp)2418
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GenBank IDAE014297.2
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Entrez GeneE2f1 (a.k.a. Dmel_CG6376, CG6376, DRTF1/E2F, DmE2F-1, Dmel\CG6376, Dp, E(Sev-CycE)3A, E(var)3-93E, E(var)3-95E, E(var)93E, E2F, E2F-1, E2F1, E2f, Evar(3)164, dE2F, dE2F1, dE2f, dE2f1, de2f1, def21, drosE2F1, e2f, e2f1, l(3)07172, l(3)j3B1, l(3)j3C2, l(3)rM729)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer M13 Forward
- 3′ sequencing primer M13 Reverse, T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR-E2F1 was a gift from Robert Duronio (Addgene plasmid # 37141 ; http://n2t.net/addgene:37141 ; RRID:Addgene_37141) -
For your References section:
Intrinsic negative cell cycle regulation provided by PIP box- and Cul4Cdt2-mediated destruction of E2f1 during S phase. Shibutani ST, de la Cruz AF, Tran V, Turbyfill WJ 3rd, Reis T, Edgar BA, Duronio RJ. Dev Cell. 2008 Dec;15(6):890-900. 10.1016/j.devcel.2008.10.003 PubMed 19081076