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Addgene

pMito-LSSmOrange
(Plasmid #37135)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37135 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pN1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4039
  • Total vector size (bp) 4749
  • Modifications to backbone
    human cytochrome C oxidase subunit VIII insert
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LSSmOrange
  • Insert Size (bp)
    710
  • Mutation
    A44V/F84L/W145M/I165D/M167L/G202D compared to mOrange (but numbering relative to EGFP)
  • Promoter CMV
  • Tag / Fusion Protein
    • human cytochrome C oxidase subunit VIII (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMito-LSSmOrange was a gift from Vladislav Verkhusha (Addgene plasmid # 37135 ; http://n2t.net/addgene:37135 ; RRID:Addgene_37135)

  • For your References section:

    An Orange Fluorescent Protein with a Large Stokes Shift for Single-Excitation Multicolor FCCS and FRET Imaging. Shcherbakova DM, Hink MA, Joosen L, Gadella TW, Verkhusha VV. J Am Chem Soc. 2012 Apr 24. 10.1021/ja3018972 PubMed 22486524
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