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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 37132 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepN1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 3959
- Total vector size (bp) 5434
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLSSmOrange-DEVD-mKate2
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Insert Size (bp)1475
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MutationA44V/F84L/W145M/I165D/M167L/G202D compared to mOrange (but numbering relative to EGFP)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLSSmOrange-mKate2 caspase-3 biosensor was a gift from Vladislav Verkhusha (Addgene plasmid # 37132 ; http://n2t.net/addgene:37132 ; RRID:Addgene_37132) -
For your References section:
An Orange Fluorescent Protein with a Large Stokes Shift for Single-Excitation Multicolor FCCS and FRET Imaging. Shcherbakova DM, Hink MA, Joosen L, Gadella TW, Verkhusha VV. J Am Chem Soc. 2012 Apr 24. 10.1021/ja3018972 PubMed 22486524
Map uploaded by the depositor.
