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Addgene

pLSSmOrange-N1 Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb DNA ladder and a digestion of plasmid 37130 with NotI-HF, BamHI-HF, and NdeI.

We observed the expected banding patterns:

NotI-HF / BamHI-HF double: expect 2 bands- 3992bp and 731bp

NotI-HF / NdeI double: expect 2 bands- 3566bp and 1157bp