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Addgene

MIB2-FLAG
(Plasmid #37117)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37117 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-3Tag-8
  • Backbone manufacturer
    Agilent (Stratagene)
  • Backbone size w/o insert (bp) 5171
  • Total vector size (bp) 8209
  • Modifications to backbone
    Replaced MCS with XhoI--NotI-A-ClaI-GTCGAG MCS sequence before vector's 3xFLAG epitopes.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MIB2, mindbomb E3 ubiquitin protein ligase 2
  • Alt name
    MIB2; ZZZ5; ZZANK1; FLJ20648; FLJ39787; FLJ41406
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3034
  • GenBank ID
    BC037542
  • Entrez Gene
    MIB2 (a.k.a. ZZANK1, ZZZ5)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3x FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV & T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MIB2-FLAG was a gift from Martin Dorf (Addgene plasmid # 37117 ; http://n2t.net/addgene:37117 ; RRID:Addgene_37117)

  • For your References section:

    Mapping a dynamic innate immunity protein interaction network regulating type I interferon production. Li S, Wang L, Berman M, Kong YY, Dorf ME. Immunity. 2011 Sep 23;35(3):426-40. Epub 2011 Sep 8. 10.1016/j.immuni.2011.06.014 PubMed 21903422
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