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Addgene

mMib1-FLAG
(Plasmid #37116)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37116 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-Tag4a
  • Backbone manufacturer
    Agilent (Stratagene)
  • Backbone size w/o insert (bp) 4319
  • Total vector size (bp) 7295
  • Modifications to backbone
    Deleted between BamHI and XhoI where ACC-Mib1 CDS is inserted.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mib1(Mus musculus mindbomb homolog 1 (Drosophila) (Mib1))
  • Alt name
    Mib; DIP-1; mKIAA1323; E430019M12Rik; MGC18948
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3021
  • Mutation
    ACC Kozak sequence added before ATG annotated.
  • GenBank ID
    NM_144860
  • Entrez Gene
    Mib1 (a.k.a. DIP-1, E430019M12Rik, Mib, mindbomb)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3 x FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mMib1-FLAG was a gift from Martin Dorf (Addgene plasmid # 37116 ; http://n2t.net/addgene:37116 ; RRID:Addgene_37116)

  • For your References section:

    Mapping a dynamic innate immunity protein interaction network regulating type I interferon production. Li S, Wang L, Berman M, Kong YY, Dorf ME. Immunity. 2011 Sep 23;35(3):426-40. Epub 2011 Sep 8. 10.1016/j.immuni.2011.06.014 PubMed 21903422
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