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Purpose(Empty Backbone)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 37114 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAK405
- Backbone size (bp) 3937
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAK405 was a gift from Julia Vorholt (Addgene plasmid # 37114 ; http://n2t.net/addgene:37114 ; RRID:Addgene_37114) -
For your References section:
Markerless gene deletion system for sphingomonads. Kaczmarczyk A, Vorholt JA, Francez-Charlot A. Appl Environ Microbiol. 2012 May;78(10):3774-7. Epub 2012 Mar 16. 10.1128/AEM.07347-11 PubMed 22427496
Map uploaded by the depositor.
