UAS::PTPRDb-DN-GFP
(Plasmid
#37112)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 37112 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDestTol2pA2
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Backbone manufacturerChi-Bien Chien
- Backbone size w/o insert (bp) 5883
- Total vector size (bp) 8655
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Vector typeTol2 Gateway Destination Vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUAS::PTPRDb-DN-GFP
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Alt nameDominant Negative PTPRDb
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)4476
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Mutationdeleted cytodomain, including phosphatase domains (aa 959-1516)
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GenBank IDJQ414009
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Entrez Geneptprdb (a.k.a. ptprd, zgc:165626)
- Promoter UAS
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer n/a
- 3′ sequencing primer M13F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
UAS::PTPRDb-DN-GFP was a gift from Alvaro Sagasti (Addgene plasmid # 37112 ; http://n2t.net/addgene:37112 ; RRID:Addgene_37112) -
For your References section:
LAR receptor tyrosine phosphatases and HSPGs guide peripheral sensory axons to the skin. Wang F, Wolfson SN, Gharib A, Sagasti A. Curr Biol. 2012 Mar 6;22(5):373-82. Epub 2012 Feb 9. 10.1016/j.cub.2012.01.040 PubMed 22326027