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UAS::PTPRFb-DN-GFP
(Plasmid #37111)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37111 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDestTol2pA2
  • Backbone manufacturer
    Chi-Bien Chien
  • Backbone size w/o insert (bp) 5883
  • Total vector size (bp) 9956
  • Vector type
    Tol2 Gateway Expression Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UAS::PTPRFb-DN-GFP
  • Alt name
    Dominant Negative PTPRFb
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    5652
  • Mutation
    Deleted aa1386-1909 corresponding to phosphatase domains 1 (active) and 2 (inactive)
  • GenBank ID
    NM_001083576
  • Entrez Gene
    ptprfb (a.k.a. ptprf, MGC154028, si:dkey-21k10.1)
  • Promoter 10xUAS
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UAS::PTPRFb-DN-GFP was a gift from Alvaro Sagasti (Addgene plasmid # 37111 ; http://n2t.net/addgene:37111 ; RRID:Addgene_37111)

  • For your References section:

    LAR receptor tyrosine phosphatases and HSPGs guide peripheral sensory axons to the skin. Wang F, Wolfson SN, Gharib A, Sagasti A. Curr Biol. 2012 Mar 6;22(5):373-82. Epub 2012 Feb 9. 10.1016/j.cub.2012.01.040 PubMed 22326027
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