Skip to main content
Addgene

pAdTrackCMV-PSM-Rb
(Plasmid #37107)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37107 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pAdTrack-CMV
  • Backbone size w/o insert (bp) 9220
  • Vector type
    Mammalian Expression, Adenoviral
  • Selectable markers
    EGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Rb
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • Mutation
    aa 379-928 only; large pocket of human Rb with aa substitutions S780A, S788D, S795A, S807A, S811L, T821A, T826A
  • Entrez Gene
    RB1 (a.k.a. OSRC, PPP1R130, RB, p105-Rb, p110-RB1, pRb, pp110)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (insert)/BglII (vector) (destroyed during cloning)
  • 3′ cloning site BamHI (insert)/BglII (vector) (destroyed during cloning)
  • 5′ sequencing primer pAd-CMV
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Also known as PSM.7LP (Knudsen and Wang 1997 MCB 17:5771).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAdTrackCMV-PSM-Rb was a gift from Joseph Nevins (Addgene plasmid # 37107 ; http://n2t.net/addgene:37107 ; RRID:Addgene_37107)

  • For your References section:

    A role for Mediator complex subunit MED13L in Rb/E2F-induced growth arrest. Angus SP, Nevins JR. Oncogene. 2012 Jan 16. doi: 10.1038/onc.2011.622. 10.1038/onc.2011.622 PubMed 22249253
Related items:
Commonly requested with: