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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 37104 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepQCXIH
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 7800
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Vector typeMammalian Expression, Retroviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCDKN2A
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Alt namep16INK4a
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SpeciesH. sapiens (human)
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Insert Size (bp)600
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GenBank IDNM_000077.4
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Entrez GeneCDKN2A (a.k.a. ARF, CAI2, CDK4I, CDKN2, CMM2, INK4, INK4A, MLM, MTS-1, MTS1, P14, P14ARF, P16, P16-INK4A, P16INK4, P16INK4A, P19, P19ARF, TP16)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (insert)/AgeI (vector) (destroyed during cloning)
- 3′ cloning site XhoI (insert)/AgeI (vector) (destroyed during cloning)
- 5′ sequencing primer LNCX
- 3′ sequencing primer IRES-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pQCXIH-CDKN2A was a gift from Joseph Nevins (Addgene plasmid # 37104 ; http://n2t.net/addgene:37104 ; RRID:Addgene_37104) -
For your References section:
A role for Mediator complex subunit MED13L in Rb/E2F-induced growth arrest. Angus SP, Nevins JR. Oncogene. 2012 Jan 16. doi: 10.1038/onc.2011.622. 10.1038/onc.2011.622 PubMed 22249253