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pBJ1807 pRS415-COF1RFP
(Plasmid #37102)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37102 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS415-LEU,Amp
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    COF1-RFP
  • Alt name
    Cofilin-RFP
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    Single RFP internal insertion at Cof147T48D with linker(AGAGDGAGL) at both sides. This construct contains endogenous COF1 5' 650 , intron, and 3' 250 bp. Cof1-RFP labels actin patches in yeast.
  • Tag / Fusion Protein
    • RFP

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBJ1807 pRS415-COF1RFP was a gift from John Cooper (Addgene plasmid # 37102 ; http://n2t.net/addgene:37102 ; RRID:Addgene_37102)

  • For your References section:

    Overlapping and distinct functions for cofilin, coronin and Aip1 in actin dynamics in vivo. Lin MC, Galletta BJ, Sept D, Cooper JA. J Cell Sci. 2010 Apr 15;123(Pt 8):1329-42. Epub 2010 Mar 23. 10.1242/jcs.065698 PubMed 20332110
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