pcDNA3 HA-PHLPP1 full length
(Plasmid #37100)

• Purpose: Expresses HA tagged, full-length human PHLPP1 in mammalian cells
• Depositing Lab: Alexandra Newton
• Publication: Warfel et al J Biol Chem. 2011 Jun 3;286(22):19777-88. Epub 2011 Mar 28.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PHLPP1
• Alt name: SCOP
• Species: H. sapiens (human)
• GenBank ID: NM_194449
• Entrez Gene: PHLPP1 (a.k.a. PHLPP, PLEKHE1, PPM3A, SCOP)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the insert in this plasmid was referred to as PHLPP1beta in the original publication (Brognard et al., Mol. Cell 2007) and the plasmid was first described in Warfel et al. 2011.