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Addgene

pcDWUER
(Plasmid #37094)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37094 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5535
  • Total vector size (bp) 7917
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    WU polyomavirus early region
  • Alt name
    LTAg/STAg
  • Species
    WU polyomavirus
  • Insert Size (bp)
    2382
  • GenBank ID
    NC_009539.1
  • Entrez Gene
    WUPyV_gp4 (a.k.a. WUPyV_gp4)
  • Entrez Gene
    WUPyV_gp5 (a.k.a. WUPyV_gp5)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDWUER was a gift from David Wang (Addgene plasmid # 37094 ; http://n2t.net/addgene:37094 ; RRID:Addgene_37094)

  • For your References section:

    Identification of a novel polyomavirus from patients with acute respiratory tract infections. Gaynor AM, Nissen MD, Whiley DM, Mackay IM, Lambert SB, Wu G, Brennan DC, Storch GA, Sloots TP, Wang D. PLoS Pathog. 2007 May 4. 3(5):e64. 10.1371/journal.ppat.0030064 PubMed 17480120
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