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Addgene

pcDKIER
(Plasmid #37093)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37093 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5535
  • Total vector size (bp) 7884
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    KI polyomavirus early region
  • Alt name
    LTAg/STAg
  • Species
    KI polyomavirus
  • Insert Size (bp)
    2349
  • GenBank ID
    NC_009238.1
  • Entrez Gene
    KPV_gp4 (a.k.a. KPV_gp4)
  • Entrez Gene
    KPV_gp5 (a.k.a. KPV_gp5)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The discovery of the KI polyomavirus was first described in the following article https://www.ncbi.nlm.nih.gov/pubmed/17287263

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDKIER was a gift from David Wang (Addgene plasmid # 37093 ; http://n2t.net/addgene:37093 ; RRID:Addgene_37093)
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