pVD300recA6
(Plasmid
#37062)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 37062 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepHSX
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Modifications to backboneSee paper
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Vector typeInducible Gc expression vector
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsSlow growth, usually needs at least 18 hours, but probably closer to 20
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameRecA
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SpeciesNeisseria gonorrhoeae (Gc)
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Entrez GenerecA (a.k.a. NGO0741)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer RecA Gc F
- 3′ sequencing primer RecA Gc R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVD300recA6 was a gift from Hank Seifert (Addgene plasmid # 37062 ; http://n2t.net/addgene:37062 ; RRID:Addgene_37062) -
For your References section:
Insertionally inactivated and inducible recA alleles for use in Neisseria. Seifert HS. Gene. 1997 Apr 1;188(2):215-20. 10.1016/S0378-1119(96)00810-4 PubMed 9133594
Map uploaded by the depositor.
