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Addgene

pLKO-shSmad4
(Plasmid #37046)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37046 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLKO.1-puro
  • Backbone manufacturer
    Weinberg Lab
  • Backbone size w/o insert (bp) 6900
  • Total vector size (bp) 6958
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Smad4
  • gRNA/shRNA sequence
    CCGGGCCATAGTGAAGGACTGTTGCCTCGAGGCAACAGTCCTTCACTATGGCTTTTTG
  • Species
    H. sapiens (human)
  • Entrez Gene
    SMAD4 (a.k.a. DPC4, JIP, MADH4, MYHRS)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pLKO.1 5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLKO-shSmad4 was a gift from Sam Thiagalingam (Addgene plasmid # 37046 ; http://n2t.net/addgene:37046 ; RRID:Addgene_37046)

  • For your References section:

    Smad signaling is required to maintain epigenetic silencing during breast cancer progression. Papageorgis P, Lambert AW, Ozturk S, Gao F, Pan H, Manne U, Alekseyev YO, Thiagalingam A, Abdolmaleky HM, Lenburg M, Thiagalingam S. Cancer Res. 2010 Feb 1;70(3):968-78. Epub 2010 Jan 19. 10.1158/0008-5472.CAN-09-1872 PubMed 20086175
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