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pBabe-puro-TGFβRII-HA
(Plasmid #37040)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37040 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBabe-puro
  • Backbone manufacturer
    Weinberg Lab
  • Backbone size w/o insert (bp) 5169
  • Total vector size (bp) 7000
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TGFβRII
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1800
  • Promoter 5'LTR
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SnaBI (destroyed during cloning)
  • 3′ cloning site SnaBI (destroyed during cloning)
  • 5′ sequencing primer pBABE5'
  • 3′ sequencing primer pBABE3'
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Bert Vogelstein, Johns Hopkins University

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert was blunt-ended and ligated into SnaBI-digested pBabe-puro.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe-puro-TGFβRII-HA was a gift from Sam Thiagalingam (Addgene plasmid # 37040 ; http://n2t.net/addgene:37040 ; RRID:Addgene_37040)

  • For your References section:

    Smad4 inactivation promotes malignancy and drug resistance of colon cancer. Papageorgis P, Cheng K, Ozturk S, Gong Y, Lambert AW, Abdolmaleky HM, Zhou JR, Thiagalingam S. Cancer Res. 2011 Feb 1;71(3):998-1008. Epub 2011 Jan 18. 10.1158/0008-5472.CAN-09-3269 PubMed 21245094
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