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Addgene

pRK5myc Rac1 wt
(Plasmid #37030)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37030 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5-myc
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4750
  • Total vector size (bp) 5350
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rac1
  • Alt name
    Isoform 1 of Rac
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    600
  • Entrez Gene
    RAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1)
  • Promoter CMV
  • Tag / Fusion Protein
    • Myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5myc Rac1 wt was a gift from Lawrence Kirschner (Addgene plasmid # 37030 ; http://n2t.net/addgene:37030 ; RRID:Addgene_37030)

  • For your References section:

    Rac1 is required for Prkar1a-mediated Nf2 suppression in Schwann cell tumors. Manchanda PK, Jones GN, Lee AA, Pringle DR, Zhang M, Yu L, La Perle KM, Kirschner LS. Oncogene. 2013 Jul 25;32(30):3491-9. doi: 10.1038/onc.2012.374. Epub 2012 Oct 8. 10.1038/onc.2012.374 PubMed 23045281
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